Identification of Anaplasma marginale long-term carrier cattle by detection of serum antibody to isolated MSP-3

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Identification of Anaplasma marginale long-term carrier cattle by detection of serum antibody to isolated MSP-3.

Rapid and accurate detection of Anaplasma marginale-infected cattle would enhance anaplasmosis control procedures and evaluation of vaccines. Current tests based on detection of antibodies in serum are not widely used for several reasons, including the occurrence of either false-positive or false-negative results. We evaluated binding of antibodies in serum to a subunit antigen isolated from A....

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molecular diagnostic of anaplasma marginale in carrier cattle

background: anaplasmosis belongs to the complex of several tick-borne diseases and can cause diseases in the livestock with high economical losses. cattle that recover from acute infection become carriers and the parasite can persist most probably for the lifetime in the blood. the aim of the present study was the determination of the persistently infected cattle in a region of iran with the pr...

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comparison of microscopy and pcr-rflp for detection of anaplasma marginale in carrier cattle

background and objectives : in iran, anaplasmosis is normally diagnosed with traditional giemsa staining method. this is not applicable for identification of the carrier animals. the aim of this study was to compare the detection of anaplasma marginale in two different numbers of microscopic fields (50 and 100) using conventional giemsa staining method compared with the pcr-rflp technique. mate...

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Comparison of Microscopy and PCR-RFLP for detection of Anaplasma marginale in carrier cattle

BACKGROUND AND OBJECTIVES In Iran, anaplasmosis is normally diagnosed with traditional Giemsa staining method. This is not applicable for identification of the carrier animals. The aim of this study was to compare the detection of Anaplasma marginale in two different numbers of microscopic fields (50 and 100) using conventional Giemsa staining method compared with the PCR-RFLP technique. MATE...

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Molecular Detection of Anaplasma marginale and Anaplasma ovis (Rickettsiales: Anaplasmataceae) in Ixodid Tick Species in Iran

The present study was conducted as the first molecular detection of Anaplasma species in tick samples based on the sequencing of major surface proteins 4 (msp4) gene fragments in different parts of Iran. A total of 130 tick specimens were collected from Hormozgan, Lorestan, and Guilan, Iran, within 2015 to 2017. Hyalomma asiaticum, Hyalomma dromedarii</em...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1991

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.29.4.788-793.1991